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CrabLab Experiments

Just some of the protocols I used in the CrabLab.

Imaging

  • Di-4 ANEPPS: 20 ┬Ál to 1ml in well around desheathed STG for 20 minutes
  • Use 20 x objective
  • One high res picture + 3 low res pictures

Dopamine experiment

  • Start with 10-6M dopamine solution.
  • 10 minutes perfusion
  • Image: 1 high res + 3 low res
  • 10-4 DA solution
  • 10 minutes perfusion
  • Image: 1 high res + 3 low res
  • Washout 15 minutes with normal saline
  • Image: 1 high res + 3 low res

Pilo

  • Cut stn
  • Image
  • Apply Pilocarpine
  • Perfuse 10 minutes
  • Image